Section

Ecology

Title

FREE RADICAL OXIDATION STATUS OF SERUM BLOOD OF AMPHIBIANS IN URBAN AREAS

Сontributors

E. B. Romanova, D. of Biol. Sc., Professor at the Lobachevsky State University of Nizhni Novgorod, This email address is being protected from spambots. You need JavaScript enabled to view it.,

K. V. Shapovalova, Postgraduate Student at the Lobachevsky State University of Nizhni Novgorod, This email address is being protected from spambots. You need JavaScript enabled to view it.,

A.V. Kozyreva, Bachelor, Graduate Student of the First Year of Study

at the Lobachevsky State University of Nizhni Novgorod, This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract

Assessment of intensity of oxidizing reactions in serums of blood of the lake and pond frogs living in reservoirs of the anthropogenous transformed urban environment on the level of the oxidizing modifi-cation of proteins (OMP) and free radical oxidation is carried out by method of the induced hemily- uminestsention. In the conditions of pollution of reservoirs (the coefficient of complexity of impurity of water varied from 25,00 to 66,67 %) indicators of the oxidized the aldegidic and ketonic amino-acid remains of proteins of serum of blood increased. High sensitivity to oxidation of proteins of serum of blood correlated with contents in the water environment of heavy metals (iron, copper, nickel). The general antioxidant activity (1/6) studied samples of serum of blood of pond and lake frogs was com-parable. For lake frogs change of activity of free radical reactions of /max (mV) and speeds of restoration of the antioxidant tg (—2a') an organism of the amphibians living in qualitatively different conditions of the environment is established. The condition of an oxidizing homeostasis of green frogs of the urbanized territory was defined by structure, concentration of priority pollutants and taxonomical accessory of animals (even within one sort). In the conditions of pollution indicators about — antioxidant balance were displaced towards an intensification of free radical oxidation and strengthening of work of antioxidant system.

Keywords

amphibians, oxidizing modification of proteins, antioxidant activity.

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